Coding

Part:BBa_K2007002:Design

Designed by: Alina Khzanyan   Group: iGEM16_ULV-LC-CV   (2016-10-10)


Drosophila melanogaster Juvenile Hormone Acid O-methyltransferase (DmJHAMT)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 133
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We introduced two silent mutations to eliminate two Pst1 cut sites by changing the base pairs at position 72 from A to G and at position 558 from A to G so that we could use this part in a standard Bio Brick format.


Source

Sherkhanov, S., Korman, T. P., Clarke, S. G., & Bowie, J. U. (2016). Production of FAME biodiesel in E. coli by direct methylation with an insect enzyme. Scientific reports, 6.

References